In recent years, heavy metals pollution is found to be a serious environmental issue and various technologies are being discovered for their cleanup from environment. The use of biosurfactants for this purpose was found to be an eco-friendly approach and also an alternate to conventional complex remediation systems. Due to their diversity, biosurfactants are considered as a potential candidate for the environmental cleanup of pollutants. Therefore, a greater attention was paid on biosurfactants and identifying their potential applications. By considering the importance of biosurfactants, the present review article presents an exhaustive evaluation of different sources of biosurfactants and also their properties and applications which is the focus of future studies.