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Research Journal of Microbiology
  Year: 2012 | Volume: 7 | Issue: 1 | Page No.: 68-74
DOI: 10.3923/jm.2012.68.74
Brucella ovis as a Common Antigen for Rapid Diagnosis of Rough Brucellosis in Cattle and Sheep
W.S. Shell, M.A. Saad, K.A. Abd El-Razik, M.L. Sayed and R.M. Shaapan

Abstract:
Brucellosis still represents a serious public health problem that results in significant morbidity and economic losses and regarded as one of the major zoonotic infections worldwide. Rough Brucella ovis strain (REO, 198) was used as antigens in routine and modified Rapid Slide Agglutination Test (RSAT) stained with Rose Bengal stain, Complement Fixation Test (CFT) and Agar Gel Precipitation Test (AGPT) for diagnosis of rough Brucella infected and vaccinated cattle and sheep in comparison with conventional RSAT incorporated with smooth S99 strain as antigen. The Rough RSAT using Brucella ovis as antigen showed a high sensitivity in identifying all experimentally B. ovis infected sheep and RB51 vaccinated cattle. Moreover, this antigen prepared with 0.1 M PBS (not 10% saline) showed negative results in 28 out of 30 naturally smooth Brucella infected sheep. On the contrary, cattle and sheep vaccinated with S19 and Rev-1 vaccines reacted positively with the rough as well as the conventional (smooth) Rose Bengal antigens. AGPT with rough antigens yielded the same results of RSAT in vaccinated RB51 cattle and experimentally B. ovis infected sheep whereas CFT was less sensitive. The current study advise the use of conventional RSAT either by modified or traditional way in parallel with the rough RSAT (prepared with 0.1 M PBS) as screening tests for routine diagnosis of ovine brucellosis followed by the AGPT as confirmatory test and moreover the rough RSAT can be used for identifying of RB51 vaccination in cattle.
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How to cite this article:

W.S. Shell, M.A. Saad, K.A. Abd El-Razik, M.L. Sayed and R.M. Shaapan, 2012. Brucella ovis as a Common Antigen for Rapid Diagnosis of Rough Brucellosis in Cattle and Sheep. Research Journal of Microbiology, 7: 68-74.

DOI: 10.3923/jm.2012.68.74

URL: https://scialert.net/abstract/?doi=jm.2012.68.74

 
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