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  2. Vol 7 (1), 2012
  3. 23-31
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Research Journal of Microbiology

Year: 2012 | Volume: 7 | Issue: 1 | Page No.: 23-31

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Authors


H.H. Azzaz

Country: Egypt

H.A. Murad

Country: Egypt

A.M. Kholif

Country: Egypt

M.A. Hanfy

Country: Egypt

M.H. Abdel Gawad

Country: Egypt

Keywords


  • A. niger
  • banana wastes
  • Cellulase production
  • nitrogen source
  • optimum conditions
  • wheat straw
Research Article

Optimization of Culture Conditions Affecting Fungal Cellulase Production

H.H. Azzaz, H.A. Murad, A.M. Kholif, M.A. Hanfy and M.H. Abdel Gawad
Local isolated Fungal cultures including Aspergillus niger, Fusarium oxysporum, Fusarium avenaceum and Cephalosporium acremonium were employed for cellulase production. The current study aimed at optimization conditions of cellulase production from our local fungal strains. These fungi were grown as stand cultures in 1000 mL conical flasks containing cellulose powder medium for screening their ability for utilizing cellulose as main carbon source for cellulase production. A. niger was chosen on the basis of the best mean cellulase activity reached 0.076 U mL-1, for optimizing culture condition for cellulase production. Wheat straw was used as a sole carbon source for the enzyme production at a concentration of 20% (w/v). The highest activity reached 0.097 U mL-1 was obtained under the optimum conditions of cellulase production including 4% inoculum size, 72 h incubation period, initial pH 6 of the growth medium with using meat extract as a sole nitrogen source at a concentration of 0.33 g L-1 . The result obtained indicated the possibility of cellulase production from A. niger local strain using wheat straw as a sole carbon source.
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How to cite this article

H.H. Azzaz, H.A. Murad, A.M. Kholif, M.A. Hanfy and M.H. Abdel Gawad, 2012. Optimization of Culture Conditions Affecting Fungal Cellulase Production. Research Journal of Microbiology, 7: 23-31.

URL: https://scialert.net/abstract/?doi=jm.2012.23.31

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