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Research Journal of Microbiology
  Year: 2011 | Volume: 6 | Issue: 12 | Page No.: 926-930
DOI: 10.3923/jm.2011.926.930
 
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Antibiofilm Activity of Bacteria Isolated from Marine Environment in Indonesia against Vibrio cholerae

D.E. Waturangi, Y.A. Bunardi and S. Magdalena

Abstract:
Cholera is a waterborne-disease caused by Vibrio cholerae Biofilm inhibition has become a new approach to overcome this disease. Several publication reported marine microorganisms have been known to produce many bioactive compounds against pathogenic bacteria. The objective of this research was to obtain isolate marine bacteria with the ability to inhibit Vibrio cholerae Biofilm formation. The antibiofilm activity was determined by measuring the optical density of crystal violet which was used to stain the Vibrio cholerae Biofilm. Eleven, out of 238 isolates from several shores in Jakarta showed antibiofilm activity. Three of the eleven isolates with higher activity (KMC10, BMA1 and BLC1) were further identified using PCR and DNA sequencing of 16S rRNA gene analysis. KMC10 showed 96% similarity with Pseudoalteromonas nigrifaciens, BMA1 showed 96% similarity with Exiguobacterium sp., while BLC1 showed 99% similarity with Vibrio gallicus. Further studies are needed to characterize the bioactive compound as potential agent for cholera treatment.
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How to cite this article:

D.E. Waturangi, Y.A. Bunardi and S. Magdalena, 2011. Antibiofilm Activity of Bacteria Isolated from Marine Environment in Indonesia against Vibrio cholerae. Research Journal of Microbiology, 6: 926-930.

DOI: 10.3923/jm.2011.926.930

URL: https://scialert.net/abstract/?doi=jm.2011.926.930

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