Apitherapy or therapy with bee products as honey is an old tradition. In this study, the antimicrobial activity of 8 honey samples were investigated against 6 bacteria potential pathogens belonging to Gram positive and Gram negative bacteria. The Gram positive bacteria were Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes and Corynebacteria pseudotuberculosis. Where the Gram negative bacteria were Klebsiella pneumonia (ATCC 27736), Pseudomonas aeruginosa(ATCC 27853) and Escherichia coli(ATCC 35218). Honey samples were obtained from 8 different floral sources. The honey samples were acacia honey, citrus honey, clover honey, coriander honey, cotton honey, palm honey and sesame honey. Beside one saudi honey sample (sider honey). The findings indicate that honey samples with different Egyptian honeys and Saudi honey sample (sider honey), were effective antibacterial against different pathogenic bacteria. Honey prevents growth of the isolates and inhibits their growth when honey was added to growing culture. The results also showed that most of honey samples at 20.30% (w/v) can completely inhibit the growth of all of the tested bacteria. It was clear that the different types of honey were less effective against E. coli than other bacteria.