
	Research Journal of Microbiology
	Year: 2011 \| Volume: 6 \| Issue: 2 \| Page No.: 140-146 DOI: 10.3923/jm.2011.140.146

Characterization of Alpha Amylase from Bacillus subtilis BS5 Isolated from Amitermes evuncifer Silvestri

T.O. Femi-Ola and B.M. Olowe

Abstract: Amylase producer Bacillus subtilis BS5 was obtained from the hindgut of wood eating termites Amitermis evuncifer Silvestri. Amylase enzyme produced by this strain when grown on basal liquid medium with cocoyam starch as substrate was characterized. The enzyme was purified through ion-exchange chromatography and gel-filtration. The purified enzyme had a molecular weight of 63 kDa and was active optimally at pH 6.0 and 50°C. The apparent K_m value and V_max of the enzyme during hydrolysis of soluble starch were 16.67 mg mL^-1 and 3.8 mg/min/mL^-1, respectively. The activities of the amylase were stimulated by Ca²⁺, Na⁺ and Mg²⁺ but inhibited by EDTA and HgCl_2.

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How to cite this article:

T.O. Femi-Ola and B.M. Olowe, 2011. Characterization of Alpha Amylase from Bacillus subtilis BS5 Isolated from Amitermes evuncifer Silvestri. Research Journal of Microbiology, 6: 140-146.

DOI: 10.3923/jm.2011.140.146

URL: https://scialert.net/abstract/?doi=jm.2011.140.146

COMMENTS

09 March, 2011
tanya tejeswita:
very clean results

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