Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2010 | Volume: 5 | Issue: 8 | Page No.: 707-716
DOI: 10.3923/jm.2010.707.716
Studies on Selection of Efficient Bacterial Strain Simultaneously Tolerant to Hexavalent Chromium and Pentachlorophenol Isolated from Treated Tannery Effluent
M. Tripathi and S.K. Garg

Abstract:
Bioremediation is being viewed as a clean technology for decontamination of pentachlorophenol and chromium from tannery effluent. This study was conducted to isolate an efficient bacterial culture from treated tannery effluent which is tolerant to pentachlorophenol and Cr (VI) and could be employed for simultaneous bioremediation of both the toxic contaminants. Tannery effluent sample was collected from Common Effluent Treatment Plant, Kanpur (India). Fifty four bacterial cultures isolated by enrichment culture technique were screened for PCP and Cr (VI) tolerance on minimal salt medium supplemented with glucose as a cometabolite. The isolate B4 was found to be maximally tolerant to high concentration of both pentachlorophenol (500 mg L-1) and chromium (VI) (200 mg L-1) and was selected for further studies. It was identified as Bacillus sp. by morphological and biochemical analyses. The effect of various growth parameters such as carbon source at 0.2-1.0% (glucose, maltose, sucrose), pH (6.5-8.0), temperature (25-40°C) and inoculum size (0.5-2.5% v/v) were evaluated in minimal salt medium supplemented with 500 mg L-1 PCP and 200 mg L-1 Cr (VI). The best growth was exhibited at 0.4 % glucose, pH 7.0, 35°C and with 1.0% inoculum under shaking at 150 rpm. Thus, our isolate appears to have great potential for simultaneous bioremediation of pentachlorophenol and hexavalent chromium from the contaminated sites.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Differential Responses of Marine Sediment Bacteria Pseudomonas stutzeri Strain VKM014 to Chromate Exposures
  •    Hexavalent Chromium Reduction in Tannery Effluent by Bacterial Species Isolated from Tannery Effluent Contaminated Soil
  •    Detoxification of Cr (VI) by Bacillus cereus S-6
How to cite this article:

M. Tripathi and S.K. Garg, 2010. Studies on Selection of Efficient Bacterial Strain Simultaneously Tolerant to Hexavalent Chromium and Pentachlorophenol Isolated from Treated Tannery Effluent. Research Journal of Microbiology, 5: 707-716.

DOI: 10.3923/jm.2010.707.716

URL: https://scialert.net/abstract/?doi=jm.2010.707.716

COMMENTS
03 August, 2011
swati sukla:
its a very good research article for bioremediation of chromium and pentachlorophenol
 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

Curve Bottom