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Research Journal of Microbiology
  Year: 2010 | Volume: 5 | Issue: 6 | Page No.: 483-489
DOI: 10.3923/jm.2010.483.489
Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli from Fish
K. Srividya, L. Anbu Rajan, Y. Rajalakshmi and V. Umashankar

Abstract:
Trehalose, a non-reducing disaccharide is a major compatible solute, maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, Trehalose-6-Phosphate Synthase (TPS) and Trehalose-6-Phosphate Phosphatase (TPP) genes encoding for trehalose biosynthesis were PCR amplified from genomic DNA of Escherichia coli isolated from Rohu (Labeo rohita) collected from local fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that TPS and TPP genes contain 1, 424 and 801 bp long ORF encoding 474 and 266 amino acids, respectively. In silico sequence analysis revealed that the TPS and TPP sequences of E. coli were conserved in many eubacteria. In conclusion, the nucleotide sequence of TPS and TPP genes of this study make known to have a high degree of similarity with other organisms. The phylogenetic tree analysis of nucleotide and amino acid sequences of TPS and TPP of our isolate also reveals the maximum similarity with other organisms.
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How to cite this article:

K. Srividya, L. Anbu Rajan, Y. Rajalakshmi and V. Umashankar, 2010. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli from Fish. Research Journal of Microbiology, 5: 483-489.

DOI: 10.3923/jm.2010.483.489

URL: https://scialert.net/abstract/?doi=jm.2010.483.489

 
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