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Research Journal of Microbiology
  Year: 2009 | Volume: 4 | Issue: 7 | Page No.: 260-268
DOI: 10.3923/jm.2009.260.268
 
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Isolation of Extreme Halotolerant Bacteria from Asian Desert Dust; Molecular Phylogeny and Growth Properties of their Cells
H. Sasaki, E. Iwata, A. Oshima, A. Ishida and S. Nagata

Abstract:
We tried the isolation of halophilic bacteria from Asian desert dust falls in Japan and growth property of these bacteria and their molecular phylogeny were analyzed. Two Gram-positive bacteria designated as IMU-1 and IMU-2 were isolated from Asian desert dust. These two strains were adapted with 0-3 and 0-4 M NaCl under nutrient medium culture conditions, respectively, showing the properties of halotolerance. Under the Davis minimal medium culture condition, IMU-1 attained to the similar level of growth as that of nutrient medium culture and growth was observed at 0-2.5 M NaCl. On the other hand, IMU-2 showed the different growth as that of nutrient medium culture condition and growth was observed at 0-1.2 M NaCl. Phylogenetic analysis using 16S rRNA gene sequences revealed that IMU-1 and IMU-2 belong to Bacillus licheniformis and B. megaterium, respectively. It was first study about the isolation of B. licheniformis as the halophilic bacteria in Japan.
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How to cite this article:

H. Sasaki, E. Iwata, A. Oshima, A. Ishida and S. Nagata, 2009. Isolation of Extreme Halotolerant Bacteria from Asian Desert Dust; Molecular Phylogeny and Growth Properties of their Cells. Research Journal of Microbiology, 4: 260-268.

DOI: 10.3923/jm.2009.260.268

URL: https://scialert.net/abstract/?doi=jm.2009.260.268

 
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