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Research Journal of Microbiology
  Year: 2009 | Volume: 4 | Issue: 7 | Page No.: 251-259
DOI: 10.3923/jm.2009.251.259
 
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Optimisation of Media and Cultivation Conditions for Alkaline Protease Production by Alkaliphilic Bacillus halodurans
Abdelnasser S.S. Ibrahim and Ali A. Al-Salamah

Abstract:
Media and cultivation conditions were investigated to optimize alkaline protease production by alkaliphilic Bacillus halodurans. This includes different carbon, nitrogen and metals sources in addition to different pH, incubation temperature and aeration level. The specific enzyme activity was increased by about 48.8 fold by optimizing different nutrient sources and cultivation conditions. The maximum specific enzyme activity was obtained in a medium containing 15 g L-1 lactose as the carbon source, 6 g L-1 soybean as the nitrogen source and a 5 mM mixture of Mg, Mn and Ca as trace elements, fermentation for 48 h at 37°C and agitation at 200 rpm. This study indicated the significance of nutrient source and cultivation conditions on the alkaline enzyme production by Bacillus halodurans.
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How to cite this article:

Abdelnasser S.S. Ibrahim and Ali A. Al-Salamah, 2009. Optimisation of Media and Cultivation Conditions for Alkaline Protease Production by Alkaliphilic Bacillus halodurans. Research Journal of Microbiology, 4: 251-259.

DOI: 10.3923/jm.2009.251.259

URL: https://scialert.net/abstract/?doi=jm.2009.251.259

 
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