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Research Journal of Microbiology
  Year: 2008 | Volume: 3 | Issue: 6 | Page No.: 466-473
DOI: 10.3923/jm.2008.466.473
An Artificial Mouth System (NAM Model) for Oral Biofilm Research
Z.H.A. Rahim, A.R. Fathilah, S. Irwan and W.I. Wan Nordini Hasnor

The objective of this study was to validate NAM model, an artificial mouth system for use in the study of oral biofilms. The NAM model consists of a cylindrical glass chamber (1x6 cm) which was used to mimic the oral cavity and glass beads (3 mm), placed along its length to provide surfaces for biofilm formation. The opening at both ends of the chamber which were fitted with rubber tubing served as an inlet to and outlet from the system. The two tubing were then connected to a reservoir (bacterial reservoir) via a peristaltic pump. The apparatus was kept at 37°C in a water bath. In the formation of oral biofilm, saliva was first pumped into the system to coat the glass beads. Excess saliva was then rinsed with distilled deionised water. Bacterial inoculum (Strep. mutans) was then allowed to flow into the system for 24 h. The bacterial population (cfu mL-1) in the biofilm developed on each of the glass beads in different experiments were analyzed and, validated for reproducibility. Its efficiency in maintaining temperature and flow rate for the experiment and sterility prior to the experiment was also determined. The results obtained showed that the bacterial counts of the biofilms between glass beads are not significantly different (p>0.6) and demonstrated reproducibility (4-5% standard deviation) between different experiments. It was also observed that the flow rate and temperature are constant and sterility of the apparatus is maintained throughout the experiment. This shows that the NAM model is valid for use in the in vitro study of oral biofilm development.
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How to cite this article:

Z.H.A. Rahim, A.R. Fathilah, S. Irwan and W.I. Wan Nordini Hasnor, 2008. An Artificial Mouth System (NAM Model) for Oral Biofilm Research. Research Journal of Microbiology, 3: 466-473.

DOI: 10.3923/jm.2008.466.473






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