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Research Journal of Microbiology
  Year: 2008 | Volume: 3 | Issue: 6 | Page No.: 414-422
DOI: 10.3923/jm.2008.414.422
 
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Antimicrobial Activity of Dichloromethane-Methanol (1:1 v/v) Extract from the Stem Bark of Coula edulis Bail. (Olacaceae)

J.D. Tamokou, J.R. Kuiate, G.S.S. Njateng, D.J. Mpetga Simo, A.J. Njouendou, P. Tane and P.H. Amvam Zollo

Abstract:
In order to confirm the traditional uses of Coula edulis, the CH2Cl2-MeOH (1:1 v/v) extract of stem bark of this plant and its column fractions were screened for antimicrobial activity. The plant was dried and extracted by maceration in CH2-Cl2-MeOH (1:1 v/v). The dry extract was fractionated by silica gel column chromatography. Phytochemical screening was performed using common chemical standard methods. Antimicrobial activity was assayed by disc diffusion method and broth macro dilution method. From the results, it appeared that the crude extract of Coula edulis stem bark displayed antibacterial activities against four clinical isolates of bacteria and antifungal activities against six strains of Candida species. The Minimum Inhibitory Concentration (MIC) values ranged from 12.5 to 25 mg mL-1 for bacteria and 1.56 to 6.25 mg mL-1 for yeasts. The fractionation of crude extract gave eight fractions. Fractions F3 and F4 showed higher antibacterial activities while fractions F5 and F6 displayed higher antifungal activity compared to the crude extract. Their MICs ranged from 0.19 to 12.5 mg mL-1. Phytochemical screening indicated that the crude extract contains tannins, flavonoids, anthraquinones, anthocyanins, sterols and phenols. Coula edulis crude extract has the ability to inhibit bacterial and yeast growth. Fractionation enhanced the antimicrobial activity in some fractions. These results justify the traditional use of this plant for the treatment of infectious diseases.
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How to cite this article:

J.D. Tamokou, J.R. Kuiate, G.S.S. Njateng, D.J. Mpetga Simo, A.J. Njouendou, P. Tane and P.H. Amvam Zollo, 2008. Antimicrobial Activity of Dichloromethane-Methanol (1:1 v/v) Extract from the Stem Bark of Coula edulis Bail. (Olacaceae). Research Journal of Microbiology, 3: 414-422.

DOI: 10.3923/jm.2008.414.422

URL: https://scialert.net/abstract/?doi=jm.2008.414.422

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