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Research Journal of Microbiology
  Year: 2008 | Volume: 3 | Issue: 4 | Page No.: 237-245
DOI: 10.3923/jm.2008.237.245
Synergistic Activities of 4-Arylcoumarins Against Phytopathogenic Fungi
Thongchai Taechowisan, Asawin Wanbanjob, Pittaya Tuntiwachwuttikul, Yuemao Shen and Saisamorn Lumyong

Abstract:
Different extracts of Streptomyces aureofaciens CMUAc130 culture were studied as potential antifungal agents for selected phytopathogenic fungi. In a serial agar dilution method, crude ethyl acetate and 10% methanol in ethyl acetate extracts exhibited fungistatic activity against Aspergillus flavus, Colletotrichum musae, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Sclerotium rolfsii. Both ethyl acetate extract and 10% methanol in ethyl acetate extract were highly effective on all tested fungi, with Minimum Inhibitory Concentration (MIC) values ranging from 0.25 to 50 and 10 to 100 mg mL-1, respectively. The major active ingredients from those extracts were purified by silica gel column chromatography and identified to be 5, 7, 4`-trimethoxy-4-phenylcoumarin (1), 4`-hydroxy-5,7-dimethoxy-4-phenylcoumarin (2), 3`-Hydroxy-5,7,4`-trimethoxy-4-phenylcoumarin (3), 5,7,3`,4`-Tetramethoxy-4-phenylcoumarin (4) and 4`-hydroxy-5,7,3`-trimethoxy-4-phenylcoumarin (5) by NMR and mass spectral data, respectively. Five compounds (1 to 5) had activity against F. oxysporum with MICs of 0.30, 1.00, 0.40, 10.00 and 20.00 mg mL-1, respectively. Compounds 1, 2 and 3 also showed a synergistic effect when combined in different concentrations, displaying four times less concentration to reach complete inhibition in the growth of F. oxysporum.
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How to cite this article:

Thongchai Taechowisan, Asawin Wanbanjob, Pittaya Tuntiwachwuttikul, Yuemao Shen and Saisamorn Lumyong, 2008. Synergistic Activities of 4-Arylcoumarins Against Phytopathogenic Fungi. Research Journal of Microbiology, 3: 237-245.

DOI: 10.3923/jm.2008.237.245

URL: https://scialert.net/abstract/?doi=jm.2008.237.245

 
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