Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2008 | Volume: 3 | Issue: 3 | Page No.: 105-113
DOI: 10.3923/jm.2008.105.113
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Comparison Among Opsonic Activity and Serum Bactericidal Activity Against Meningococci in Rabbit Sera from Vaccines After Immunization with Outer Membrane Vesicle of Neisseria meningitidis Serogroup B

Q. Behzadiyannejad, S.D. Siadat, M. Kheirandish, B. Tabaraiee, H. Ahmadi, D. Norouzian, S. Najar Peerayeh, M. Nejati and M.H. Hedayati

Abstract:
Production of effective vaccine formulations is dependent on the availability of assays for the measurement of protective immune responses. Antibody- and complement-mediated phagocytosis is the main defense mechanism against Neisseria meningitidis. Therefore, a newly developed phagocytosis assay based on flow cytometry (flow assay) and the Serum Bactericidal Activity (SBA) assay were using sera obtained from rabbit postvaccination with the Outer Membrane Vesicles (OMVs) of Neisseria meningitidis serogroup B was done in order to evaluation of the potential efficacy of (experimental) meningococcal vaccines. The OMVs were injected intramuscularly into of rabbits with boosters on days 14, 28 and 42 after the primary immunization. Phagocytic function of and intracellular oxidative burst generation by rabbit PMN, against Neisseria meningitidis serogroup B, was measured with flow cytometer (Coulter Epics-XL-Profile USA), using dihydrorhodamine-123 as probes, respectively. SBA titers are given as reciprocal Log 2 values of the dilution giving at least 50% killing of the inoculum measured as colony forming units. The results of SBA titers and quantitative flow cytometric analysis of rabbit PMN function in hyperimmun sera with the OMVs revealed a highly significant increase in opsonophagocytic responses and bactericidal antibody against serogroup B meningococci after 56 day (p< 0.05). Both SBA and opsonic activity are crucial for the protection against meningococcal disease. In conclusion, we have shown a very high correlation between opsonic activity and SBA (r = 0.91). Present results indicated that the OMVs could be as a candidate for vaccine toward serogroup B meningococci.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Q. Behzadiyannejad, S.D. Siadat, M. Kheirandish, B. Tabaraiee, H. Ahmadi, D. Norouzian, S. Najar Peerayeh, M. Nejati and M.H. Hedayati , 2008. Comparison Among Opsonic Activity and Serum Bactericidal Activity Against Meningococci in Rabbit Sera from Vaccines After Immunization with Outer Membrane Vesicle of Neisseria meningitidis Serogroup B. Research Journal of Microbiology, 3: 105-113.

DOI: 10.3923/jm.2008.105.113

URL: https://scialert.net/abstract/?doi=jm.2008.105.113

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom