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Research Journal of Microbiology

Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 74-80
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Authors

Sahar El-Shatoury

Hesham Abdulla

Ahmed Dewedar

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Research Article

Factorial Design for Optimization of Rice Straw Incorporation into Soil Using Micromonospora chalcea

Sahar El-Shatoury, Hesham Abdulla and Ahmed Dewedar
A Box-Wilson central composite design was applied to study the effect of Micromonospora chalcea inoculum-size, municipal sludge and rice straw ratios on decomposition of the straw in sandy soil. The experiment included 20 runs with five levels for each of the three factors. Results indicated highly significant effect of sludge (p = 0.005) on the straw decomposition and organic carbon release. Although inoculum size of M. chalcea was not significantly effective, it showed a significant interaction with sludge (p = 0.021). Probably, indicating a critical importance of sludge as source of essential nutrients for supporting M. chalcea growth. Extracellular enzyme profiling of M. chalcea revealed a general wide activity including: polysaccharide hydrolases, proteases, lipases, phosphatases and amino-peptidases. Response surface methodology was employed for the optimization of rice straw decomposition by M. chalcea. Conditions that supported highest carbon release were incorporating 100 g straw with 40 g sludge into 100 g sandy soil, leading to 10 folds reduction in time of straw decomposition compared to that before optimization. On the other hand, sludge ratios above 81% have retarded the decomposition process even under straw ratio as low as 10%.
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How to cite this article

Sahar El-Shatoury, Hesham Abdulla and Ahmed Dewedar, 2007. Factorial Design for Optimization of Rice Straw Incorporation into Soil Using Micromonospora chalcea . Research Journal of Microbiology, 2: 74-80.

URL: https://scialert.net/abstract/?doi=jm.2007.74.80

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