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Research Journal of Microbiology
  Year: 2007 | Volume: 2 | Issue: 10 | Page No.: 705-716
DOI: 10.3923/jm.2007.705.716
Optimised Production of Biosurfactant by Serratia marcescens DT-1P
Rajkumar Bidlan, N. Deepthi, N.K. Rastogi and H.K. Manonmani

Abstract:
The cultural conditions for the production of biosurfactant by Serratia marcescens DT-1P were optimised by Response Surface Methodology (RSM). A study of complex interactions among glycerol (1-5% w/v), inoculum (1-10 OD600), pH (4-8), temperature (20-40°C) and (NH4)2SO4 (0.5-2.0%) were carried out using central composite rotatable design of experiments. The optimum conditions, which were obtained i.e., glycerol (3.4% w/v), inoculum (1.0 OD600), pH (5.8), temperature (26.6°C) and (NH4)2SO4 (0.2%) yielded 6.0 mg L-1 biomass and glycerol (5% w/v), inoculum (10.0 OD600), pH (8.0), temperature (20.0°C) and (NH4)2SO4 (0.05%), yielded 24.48 mg % of biosurfactant. The surfactant was identified as arabinolipid.
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How to cite this article:

Rajkumar Bidlan, N. Deepthi, N.K. Rastogi and H.K. Manonmani, 2007. Optimised Production of Biosurfactant by Serratia marcescens DT-1P. Research Journal of Microbiology, 2: 705-716.

DOI: 10.3923/jm.2007.705.716

URL: https://scialert.net/abstract/?doi=jm.2007.705.716

 
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