
	Research Journal of Microbiology
	Year: 2007 \| Volume: 2 \| Issue: 9 \| Page No.: 664-670 DOI: 10.3923/jm.2007.664.670

Application of Box-Behnken Design for Optimization of Poly-γ-Glutamic Acid Production by Bacillus licheniformis SAB-26

Yasser R. Abdel-Fattah, Nadia A. Soliman and Mahmoud M. Berekaa

Abstract: Optimization of poly-γ-glutamic acid (PGA) produced by the glutamate-independent Bacillus licheniformis SAB-26 has been carried out using response surface methodology. Predicted maximum PGA yield (57.9 g L^-1) was calculated using non-linear optimization algorithm powered by Microsoft Excel, where the optimized medium composition was (g L^-1): casein hydrolysate, 12.2; ammonium sulfate, 20; K₂HPO₄, 27 and KH₂PO₄, 27 after 72 h. A verification experiment was carried out to compare predicted and experimental results, where experimental yield reached (59.9 g L^-1) in a good sign of model validity. Pulsed feeding experiment of citrate at different time intervals revealed increasing the polymer production to 88 g L^-1.

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How to cite this article:

Yasser R. Abdel-Fattah, Nadia A. Soliman and Mahmoud M. Berekaa, 2007. Application of Box-Behnken Design for Optimization of Poly-γ-Glutamic Acid Production by Bacillus licheniformis SAB-26. Research Journal of Microbiology, 2: 664-670.

DOI: 10.3923/jm.2007.664.670

URL: https://scialert.net/abstract/?doi=jm.2007.664.670

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