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Research Journal of Microbiology

Year: 2007 | Volume: 2 | Issue: 5 | Page No.: 486-490
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Authors

Jung Hun Lee

Seung Jin Bae

Jae Jin Lee

Il Kwon Bae

Seok Hoon Jeong

Eui Suk Sohn

Yeong Seon Lee

Sang Hee Lee

Keywords

In our proposed nomenclature system including GES-type (Guiana-Extended-Spectrum β-lactamase) or IBC-type (Integron-Borne Cephalosporinase) extended-spectrum β-lactamases (ESBLs), GES-2 has an amino acid substitution of G170N, GES-3 has two M62T and E104K substitutions, GES-4 has three M62T, E104K and G170S substitutions, GES-5 has a G170S substitution, GES-6 has two F104K and G170S substitutions, GES-7 (IBC-1) has a G104K substitution, GES-8 (IBC-2) has a A125L substitution and GES-9 has a G243S substitution, compared to the sequence of GES-1. This proposed nomenclature update would not confuse microbiologists studying GES-type ESBLs, fundamentally preventing misleading nomenclature of these antibiotic resistance genes. The definitive renaming of GES/IBC-type ESBLs can help also some researchers to correctly designate new GES-type ESBLs such as novel enzymes identified from some nationwide surveys. In order to detect rapidly and exactly GES-type ESBLs with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing, we also proposed here the new four key amino acid substitutions and four GES-type variants as follows: (i) GES-1 able to hydrolyze ceftazidime, (ii) GES-4 able to hydrolyze ceftazidime, cefoxitin and imipenem and able to show inhibitor-resistance, (iii) GES-5 able to hydrolyze ceftazidime, cefoxitin and imipenem and (iv) GES-9 able to hydrolyze ceftazidime and aztreonam.
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How to cite this article

Jung Hun Lee, Seung Jin Bae, Jae Jin Lee, Il Kwon Bae, Seok Hoon Jeong, Eui Suk Sohn, Yeong Seon Lee and Sang Hee Lee, 2007. Four Exact GES Variants Used for Identification of GES-type Extended-spectrum β-lactamases with Different Hydrolysis Profiles and Inhibitor-Resistance Patterns by Pyrosequencing . Research Journal of Microbiology, 2: 486-490.

URL: https://scialert.net/abstract/?doi=jm.2007.486.490

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