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Research Journal of Microbiology

Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 24-33
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Authors

Ravindra B. Malabadi

S. Raghvendra

S. Vijay Kumar

Keywords

Research Article

Production of Cellulase-Free Xylanase from a Novel Yeast Strain Used for Biobleaching in Paper Industry

Ravindra B. Malabadi, S. Raghvendra and S. Vijay Kumar
An investigation was conducted to explore the microbial xylanases that are thermostable and cellulase-free are generally preferred for biobleaching of paper pulp. A novel yeast strain, Pichia pinus isolated from a decaying wood produced up to 545 IU mL-1 of xylanolytic enzymes when grown on a medium containing 4% oat spelt xylan. The crude xylanase preparation showed no activity towards cellulolytic substrates but low levels of β-xylosidase (0. 1 IU mL-1) and α-L-arabinofuranoside (0.05 IU mL-1) were detected. The temperature and pH optima for the crude xylanase preparation were 55°C and 4.5, respectively. The crude xylanase produced mainly xylose from xylan within 10 min. Prolonged hydrolysis of xylan produced xylobiose and arabinose, in addition to xylose, as the end products. The culture filtrates of this isolate grown on oat spelt xylan or sugarcane bagasse containing 600 UL-1 enzyme could bleach sugarcane bagasse pulp in 60 min treatment at 55°C resulting in a 40% reduction in chlorine consumption and a ten point reduction in kappa number. The potential industrial applications of such cellulase-free xylanase, especially from yeast strains having activity at neutral pH, will be attractive in the paper and pulp industries to improve paper pulp quality as well as to minimize environmental pollution which occurs due to the use of hazardous chemicals by these industries.
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How to cite this article

Ravindra B. Malabadi, S. Raghvendra and S. Vijay Kumar, 2007. Production of Cellulase-Free Xylanase from a Novel Yeast Strain Used for Biobleaching in Paper Industry . Research Journal of Microbiology, 2: 24-33.

URL: https://scialert.net/abstract/?doi=jm.2007.24.33

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