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Research Journal of Microbiology

Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 13-23
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Authors

Yasser R. Abdel-Fattah

Ehab R. El-Helow

Khaled M. Ghanem

Walid A. Lotfy

Keywords

Statistically based experimental designs were applied to optimize the production of avicelase by a recently isolated endospore forming strain, WL1, that showed 99.8% 16S rDNA identity to Geobacillus stearothermophilus. Seven culture parameters were examined for their significance as effectors of avicelase expression using the Plackett-Burman factorial design. Concentrations of avicel, yeast extract and ammonium sulfate were the most significant factors affecting the process of enzyme production. The second optimization step was to figure out the levels of these three independent variables that generate maximum avicelase activity, using the Box-Behnken design. Maximal enzyme activity (0.8 U mL-1), which is approximately two folds the activity expressed in the basal medium, has been predicted at concentrations of (g L-1): Avicel (42), yeast extract (3.6) and ammonium sulfate (0.8). A verification experiment was accomplished and revealed approximately 99% model validity.
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How to cite this article

Yasser R. Abdel-Fattah, Ehab R. El-Helow, Khaled M. Ghanem and Walid A. Lotfy, 2007. Application of Factorial Designs for Optimization of Avicelase Production by a Thermophilic Geobacillus Isolate . Research Journal of Microbiology, 2: 13-23.

URL: https://scialert.net/abstract/?doi=jm.2007.13.23

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