Isolation and Identification of Lactobacilli from Garris, a Sudanese Fermented Camel’s Milk Product


	Research Journal of Microbiology
	Year: 2007 \| Volume: 2 \| Issue: 2 \| Page No.: 125-132 DOI: 10.3923/jm.2007.125.132

Isolation and Identification of Lactobacilli from Garris, a Sudanese Fermented Camel’s Milk Product

Abdel Moneim E. Sulieman , Ro Osawa and Roumiana Tsenkova

Abstract: In the present study, microbiological quality of Garris, a traditional fermented camel’s milk product obtained from two production sites in western and central Sudan was investigated. The microbiological analyses showed that Butana Garris was found to contain relatively high counts of lactobacilli (8.22±0.28 log₁₀cfu mL^-1) when compared with the counts found in Kordufan Garris (7.85±0.45 log₁₀ cfu mL^-1). However, Kordufan Garris contained relatively higher counts of yeasts (8.42±0.55 log₁₀ cfu mL^-1) when compared with that found in Butana Garris which contained 7.65±0.32 log₁₀ cfu mL^-1). The coliforms averaged 3.2±0.21 and 3.5±0.14 log₁₀ cfu mL^-1) in Kordufan and Butana Garris, respectively. Twenty strains of lactic acid bacteria were isolated from the fermented milk products and identified based on Polymerase Chain Reaction (PCR) method as belonging to the genus lactobacillus. Rapid and reliable two-step multiplex PCR assays were used to identify Garris lactobacilli at species level. A multiplex PCR was used for grouping lactobacilli with a mixture of group-specific primers followed by four multiplex PCR assays with four sorts of species-specific primer mixtures for identification at the species level. Primers used were designed from nucleotide sequences of the 16S-23S rRNA intergenic spacer region and its flaking 23S rRNA gene of members of the genus Lactobacillus registered at the GeneBank and DNA Data Bank of Japan. Five lactobacilli isolates from Kordufan Garris were identified as Lactobacillus plantarum, three isolates as Lactobacillus paracasei and two not determined. As for the Butana LAB isolates, five isolates were identified as Lactobacillus paracasei subsp paracasei, two as Lactobacillus plantarum and three were not determined.

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How to cite this article:

Abdel Moneim E. Sulieman , Ro Osawa and Roumiana Tsenkova , 2007. Isolation and Identification of Lactobacilli from Garris, a Sudanese Fermented Camel’s Milk Product . Research Journal of Microbiology, 2: 125-132.

DOI: 10.3923/jm.2007.125.132

URL: https://scialert.net/abstract/?doi=jm.2007.125.132

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