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Research Journal of Microbiology

Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 1-12
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Walid A. Lotfy

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Research Article

Production of Cephalosporin C by Acremonium chrysogenum Grown on Beet Molasses: Optimization of Process Parameters Through Statistical Experimental Designs

Walid A. Lotfy
In this research, cephalosporin C (CPC) production on pilot scale fermenters of 600 L capacity with 350 L working volume by Acremonium chrysogenum UMIP 2566.04 was performed. The effects of fermentation medium composition, inoculum concentration, initial pH and aeration rate on CPC production was investigated by using Response Surface Methodology (RSM). The Plackett-Burman design which involves two concentrations of each nutrient was effective in searching for the major medium components promoting CPC production. Under our experimental conditions; Soya oil, beet molasses and corn steep liquor were found to be the major factors contributing to the antibiotic production. Subsequently, a Box-Behnken design was used for outlining the concentration of the most effective medium constituents. Estimated optimum composition for the production of CPC was as follows: soya oil, 40 g L-1; beet molasses, 180 g L-1 and corn steep liquor, 330 g L-1. The central composite design was used for outlining the optimum values of the fermentation parameters. Estimated optimum values for the production of CPC are as follows: inoculum level, 105.5 spores mL-1; initial pH, 4.3 and aeration rate, 9364 L min-1.
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How to cite this article

Walid A. Lotfy, 2007. Production of Cephalosporin C by Acremonium chrysogenum Grown on Beet Molasses: Optimization of Process Parameters Through Statistical Experimental Designs . Research Journal of Microbiology, 2: 1-12.

URL: https://scialert.net/abstract/?doi=jm.2007.1.12

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