Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 1-12
DOI: 10.3923/jm.2007.1.12
Production of Cephalosporin C by Acremonium chrysogenum Grown on Beet Molasses: Optimization of Process Parameters Through Statistical Experimental Designs
Walid A. Lotfy

Abstract:
In this research, cephalosporin C (CPC) production on pilot scale fermenters of 600 L capacity with 350 L working volume by Acremonium chrysogenum UMIP 2566.04 was performed. The effects of fermentation medium composition, inoculum concentration, initial pH and aeration rate on CPC production was investigated by using Response Surface Methodology (RSM). The Plackett-Burman design which involves two concentrations of each nutrient was effective in searching for the major medium components promoting CPC production. Under our experimental conditions; Soya oil, beet molasses and corn steep liquor were found to be the major factors contributing to the antibiotic production. Subsequently, a Box-Behnken design was used for outlining the concentration of the most effective medium constituents. Estimated optimum composition for the production of CPC was as follows: soya oil, 40 g L-1; beet molasses, 180 g L-1 and corn steep liquor, 330 g L-1. The central composite design was used for outlining the optimum values of the fermentation parameters. Estimated optimum values for the production of CPC are as follows: inoculum level, 105.5 spores mL-1; initial pH, 4.3 and aeration rate, 9364 L min-1.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Rifamycin SV Production Using Cereal Bran as Solid Substrate with Amycolatopsis mediterranei
How to cite this article:

Walid A. Lotfy , 2007. Production of Cephalosporin C by Acremonium chrysogenum Grown on Beet Molasses: Optimization of Process Parameters Through Statistical Experimental Designs . Research Journal of Microbiology, 2: 1-12.

DOI: 10.3923/jm.2007.1.12

URL: https://scialert.net/abstract/?doi=jm.2007.1.12

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom