Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2006 | Volume: 1 | Issue: 5 | Page No.: 401-408
DOI: 10.3923/jm.2006.401.408
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Biodegradation of Crude Oil by Nitrogen Fixing Marine Bacteria Azotobacter chroococcum

R. Thavasi, S. Jayalakshmi , T. Balasubramanian and Ibrahim M. Banat

Abstract:
The present study deals with the hydrocarbon degrading potential of a marine nitrogen fixing bacterium Azotobacter chroococcum isolated from Tuticorin harbor (Lat. 08o45`N; Long. 78o13`E). Degradation of crude oil (58%) and emulsification (D610) of waste motor oil (1.51), crude oil (1.43), pea nut oil (1.39), diesel (0.69), kerosene (0.81), Naphthalane (0.36), Anthracene (0.33) and xylene (0.42) indicated its potentiality in utilization of various hydrocarbons. Growth of Azotobacter chroococcum in a mineral medium with 0.5% crude oil as sole carbon source resulted maximum cell density at 96 h with an OD value of 0.333. At the end of 96 h, the cell count was 4.5x109 CFU mL-1 and 2.5 mg mL-1 of biomass. The biosurfactant production was found to be 1 mg mL-1 at 96 h. The total nitrogen fixed was 4.2 mg L-1. Two plasmids were found with molecular weight of 4788 and 2400 base pairs, respectively. Loss of biodegradation and biosurfactant production after plasmid curing was observed, which confirmed that, the biosurfactant production and biodegradation process were plasmid mediated. Results of the present study revealed the possibility of using marine nitrogen fixing hydrocarbon degrading bacteria and their biosurfactants in the abatement of marine oil pollution.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Preliminary Study on Biodegradation of Nigerian Natural Bitumen
  •    Isolation and Characterisation of Polycyclic Aromatic Hydrocarbon Degrading Soil Microbes from Automobile Workshop Sediments
  •    Actinomycetes Community from Starch Factory Wastewater
  •    Development of Low Cost Medium for the Production of Biosurfactants
How to cite this article:

R. Thavasi, S. Jayalakshmi , T. Balasubramanian and Ibrahim M. Banat , 2006. Biodegradation of Crude Oil by Nitrogen Fixing Marine Bacteria Azotobacter chroococcum . Research Journal of Microbiology, 1: 401-408.

DOI: 10.3923/jm.2006.401.408

URL: https://scialert.net/abstract/?doi=jm.2006.401.408

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom