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Journal of Fisheries and Aquatic Science
  Year: 2018 | Volume: 13 | Issue: 2 | Page No.: 56-65
DOI: 10.3923/jfas.2018.56.65
 
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IGS-Fingerprinting and Virulence Properties of Vibrio alginolyticus Isolated from Farmed Fishes in Saudi Coast of Arabian Gulf
E.A. Noureldin , Mohamed A. Hassan and Ghamri H. Al Ramadhan

Abstract:
Background and Objective: Vibrio sp. are a crucial constituent of marine ecosystems worldwide. Vibrio alginolyticus is extremely significant and major pathogenic agent facing wild and farmed fishes. The main objective of this study was to record the molecular characterization (IGS-Typing pattern) by single PCR step and virulence genes in V. alginolyticus isolates. Materials and Methods: For this study 360 fishes were collected, including gilt head seabream (Sparus auratus), two bar seabream (Acanthopagrus bifasciatus), black bream (Acanthopagrus latus), red sea seabream (Diplodus noct), brown-spotted grouper (Epinephilus coioides) and rabbitfish (Siganus canaliculatus) between July, 2015-June, 2016. Primary identification of isolates was done conventionally using API 20NE systems (Biomerieux), while confirmation was done by sequencing of 16S rDNA and V. alginolyticus specific gene targets, construction of phylogenetic tree using Molecular Evolutionary Genetics Analysis (MEGA) 6.0 software. IGS-Typing and antibiogram test screening were also carried out for isolates. Results: Two hundred and eighty isolates of V. alginolyticus were recovered from farmed fishes. Clinical signs expressed as general septicaemia, ulcers and corneal opacity. Conventional methods identified isolates as V. alginolyticus (ID% 96-99.9), sequencing and phylogenetic tree confirmed this identification. The PCR results for IGS-Typing were expressed as four typical band patterns ≈ 420, 580, 650 and 820 bp, while those for virulence genes were positive amplification for collagenase, toxR and ompK, while tlh and VPI virulence genes were not recognized. Conclusion: Isolated V. alginolyticus were highly pathogenic to farmed fishes as determined by virulence genes identified. Results of IGS-Typing facilitate further identification of isolates by single PCR step as it records the pattern for V. alginolyticus.
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How to cite this article:

E.A. Noureldin, Mohamed A. Hassan and Ghamri H. Al Ramadhan, 2018. IGS-Fingerprinting and Virulence Properties of Vibrio alginolyticus Isolated from Farmed Fishes in Saudi Coast of Arabian Gulf. Journal of Fisheries and Aquatic Science, 13: 56-65.

DOI: 10.3923/jfas.2018.56.65

URL: https://scialert.net/abstract/?doi=jfas.2018.56.65

 
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