• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Journal of Environmental Science and Technology
  2. Vol 10 (5), 2017
  3. 230-237
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Journal of Environmental Science and Technology

Year: 2017 | Volume: 10 | Issue: 5 | Page No.: 230-237
DOI: 10.3923/jest.2017.230.237

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 201

Authors


Magda Ibrahim Soliman

Country: Egypt

Amira AbdAllah Ibrahim

Country: Egypt

Mohamed Ahmed  El-Metwally

Mohamed Ahmed El-Metwally

LiveDNA: 20.359

Diana Adel Eshak

Country: Egypt

Keywords


  • mitotic index
  • Vicia faba
  • antioxidants
  • biological control
  • Cytotoxicity effects
  • Trichoderma harzianum
  • Trichoderma viride
Research Article

Cytotoxicity Effects of Biological Control and Antioxidants using Vicia faba Chromosomal Aberration Assay

Magda Ibrahim Soliman, Amira AbdAllah Ibrahim, Mohamed Ahmed El-Metwally Mohamed Ahmed  El-Metwally's LiveDNA and Diana Adel Eshak
Background and Objective: Biological control refers to purposeful utilization of introduced or resident living organisms other than disease resistant host. Antioxidants protect cells against the damaging effects of reactive oxygen species otherwise called, free radicals such as singlet oxygen, super oxide, peroxyl radicals, hydroxyl radicals and peroxynite which results in oxidative stress leading to cellular damage. Cytological abnormalities in Vicia faba, studies are carried out in root tips as the using of this area in induction of chromosomal aberrations is one of the oldest, simplest, most reliable and inexpensive method. Therefore, this study aims to determine the cytotoxic and mutagenic effect using chromosome aberration assay (Vicia faba). Materials and Methods: Vicia faba (broad bean) seeds (2n = 12 chromosome) (Cultivar: Giza 716), were obtained from the Agriculture Research Center, Crop Research Section, Sakha, Kafr El-Sheikh, Egypt. The seeds were prepared into slides via serial steps of fixation and squashing. Data of different treated groups of root tips were represented as Mean±SE and statically analyzed using t-test to determine the significance of the differences between treatments and control at the 0.05 level of probability. Results: Results showed the highest percentage of MI% was 23.77% at 20 mM of citric acid for 24 h, while the lowest percentage was 9.54% at H2O+Trichoderma viride for 48 h. The highest percentage of abnormal mitosis was 43.53 and 37.24% at 24 h for 20 mM of (Aa+T. harzianum) and 3 mM of salicylic acid, respectively compared with control sample 9.37%, while the highest abnormal mitosis percentage at 48 h was 64.93 and 43.13% for (H2O+T. viride) and 20 mM citric acid. Conclusion: It was concluded that Trichoderma can be used safely as bio-control agents against pathogenic microbes. To use bioagents like antioxidants especially ascorbic acid and bio-control fungi especially T. harzianum in future in replacement of chemical fungicides (unsafe and inexpensiveness).
PDF Fulltext XML References Citation

How to cite this article

Magda Ibrahim Soliman, Amira AbdAllah Ibrahim, Mohamed Ahmed El-Metwally and Diana Adel Eshak, 2017. Cytotoxicity Effects of Biological Control and Antioxidants using Vicia faba Chromosomal Aberration Assay. Journal of Environmental Science and Technology, 10: 230-237.

DOI: 10.3923/jest.2017.230.237

URL: https://scialert.net/abstract/?doi=jest.2017.230.237

Related Articles

Seed-Borne Pathogens of Faba Bean in Egypt: Detection and Pathogencity
The Mutagenic Potentialities of Some Herbicides Using Vicia faba as a Biological System

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved