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Journal of Environmental Science and Technology
  Year: 2013 | Volume: 6 | Issue: 3 | Page No.: 130-137
DOI: 10.3923/jest.2013.130.137
 
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Isolation, Identification and Characterization of p, p’-DDT Degrading Bacteria from Soil

Geetika Pant, Sandeep Kumar Mistry and G. Sibi

Abstract:
DDT (dichlorodiphenyltrichloroethane), one of the organochlorine pesticides was widely used in agriculture and healthcare. Though its usage is banned in most of the countries, DDT residues cause varying negative effects through bioaccumulation and bioamagnification. Biodegradation is an potential method to detoxify the recalcitrant compounds and this study is an investigation to isolate and characterize the p, p’-DDT degrading bacteria from DDT contaminated soil. An p, p’-DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] degrading bacterium was isolated and identified by 16S rRNA studies. The role of temperature, pH and DDT concentration in the degrading ability of the isolate has also been investigated. Based on the analysis of the phenotype, biochemical characteristics and 16S rRNA, the strain was identified to belong to the bacterial genera Bacillus and was named as Bacillus strain GSS. The isolate had an optimum pH of 7.0 at 35°C and was able to degrade DDT at a wide range of concentrations with complete degradation of the DDT (10 mg L-1) and 89.3% of 15 mg L-1 in 120 h, whereas 100% degradation of 5 mg L-1 concentration was observed within 48 h. Significant degradation was observed at 72 h and 96 h for 15 and 20 mg L-1, respectively. At the end of 120 h, 73% of 20 mg L-1 and 34% of 25 mg L-1 was recorded by the isolate Bacillus strain GSS.
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How to cite this article:

Geetika Pant, Sandeep Kumar Mistry and G. Sibi, 2013. Isolation, Identification and Characterization of p, p’-DDT Degrading Bacteria from Soil. Journal of Environmental Science and Technology, 6: 130-137.

DOI: 10.3923/jest.2013.130.137

URL: https://scialert.net/abstract/?doi=jest.2013.130.137

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