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Journal of Environmental Science and Technology
  Year: 2010 | Volume: 3 | Issue: 1 | Page No.: 18-34
DOI: 10.3923/jest.2010.18.34
 
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Lignocellulolytic Enzymes and Substrate Utilization During Growth and Fruiting of Pleurotus ostreatus on Some Solid Wastes

A.A. Sherief, A.B. El-Tanash and A.M. Temraz

Abstract:
A commercial strain of Pleurotus ostreatus was cultivated on rice straw and sawdust in plastic bags. Lignin biodegradation appeared to be doubled in case of sawdust (36%) compared with rice straw (18%). However, hemicellulose and cellulose degradation were faster in case of rice straw (66.6%), (31.2%) than saw dust (60.0%), (18.2%), respectively. Free sugars appeared to be used variably during Pleurotus ostreatus growth and residual sugars as well as total protein were considerably accumulated after fruiting. In spite of fruiting of Pleurotus ostreatus on rice straw was earlier than sawdust; however, the levels of enzyme production and activities including; exoglucanase, endoglucanase, CMCase and pectinase appeared to be higher in case of using sawdust than rice straw in both of two stages namely; mycelial and fruiting stages. On contrary, xylanase showed early rate of higher production of enzyme activity on sawdust (21.0I U g-1) during mycelial growth then sharply decreased at fruiting stage (11.0I U g-1). In addition Mn-peroxidase (MnPase) activity was more or less equal on both of the two wastes.
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How to cite this article:

A.A. Sherief, A.B. El-Tanash and A.M. Temraz, 2010. Lignocellulolytic Enzymes and Substrate Utilization During Growth and Fruiting of Pleurotus ostreatus on Some Solid Wastes. Journal of Environmental Science and Technology, 3: 18-34.

DOI: 10.3923/jest.2010.18.34

URL: https://scialert.net/abstract/?doi=jest.2010.18.34

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