Abstract: Background and Objective: A chemical stimulus might cause a mild repulsive reaction which can be used to train an animal to avoid a cue. Repeated training may be able to induce associative learning to an unconditioned stimulus (US). The retention (memory) of the US would likely be stronger for more sensitive animals. Materials and Methods: A genetically engineered strain of Drosophila which contains inserted genes to code for the TRPV receptor (i.e., the capsaicin or heat receptor) were used to examine this topic. Results: The background control (UAS-TRPV1 strain) did show a preference to light or dark. However, associative learning to fructose (FRU) or quinine hemisulfate (QUI) was only demonstrated in 2nd instars. The transgenic larvae are extremely sensitive to capsaicin while background strain show no aversion to the capsaicin. There is preference for the dark in both background and TRPV1 expressing larvae. The background and TRPV1 receptor expressing larvae did not show associative learning to a single exposure of capsaicin. Conclusion: Even though there is a strong response to a noxious stimulus, this learned behavior is not retained when associated with non-noxious stimulus.