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Journal of Entomology

Year: 2016 | Volume: 13 | Issue: 5 | Page No.: 179-186
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Authors

Mojtaba Esmaeily

Ali Reza Bandani

Keywords

Research Article

Inhibition of Larvae and Adult α-amylase in Xanthogaleruca luteola Muller (Col.: Chrysomelidae) by Cereal and Legumes Seed Proteinaceous Extracts

Mojtaba Esmaeily and Ali Reza Bandani
Background and Objective: The elm leaf beetle, Xanthogaleruca luteola Muller (Coleoptera: Chrysomelidae) is one of the most important municipal insect pests of elm trees (Ulmus spp.) in Iran. Aim of this study was investigating effects of proteinaceous extracts of cereal and legume seeds to the X. luteola α-amylase in in vitro condition. Materials and Methods: Gut enzymes were extracted using sodium phosphate buffer and seed proteinaceous compounds were extracted using 0.1 M NaCl. The α-amylase activity assay was performed using the dinitrosalicylic acid (DNS) procedure. Results: The results showed that mung bean, pea, broad bean, barley, triticale, alborz and kavir inhibited the enzyme activity of insect. Kavir had the best inhibition activity with 90 and 84% of inhibition, respectively. Conclusion: Inhibitors of mung bean and kavir seeds have a good potential to be use in plant protection. So, it could be included that these inhibitors are more promising candidates for this pest control and decrees the chemical insecticide with adverse effect in human health.
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