Submit Manuscript

Journal of Entomology

Year: 2014 | Volume: 11 | Issue: 1 | Page No.: 14-24
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 131

Authors

El-Sayed A. El-Sheikh

Mohamed-Bassem A. Ashour

Mohamed M. Aamir

Mohamed M. Gamal

Keywords

The susceptibility of Culex pipiens collected from different localities of the Sharkia Governorate, Egypt, to malathion and lambda-cyhalothrin was investigated for 11 successive generations. Larval Cx. pipiens developed 57 and 305-fold resistance to malathion and lambda-cyhalothrin, respectively after 11 successive generations of selection pressure. The susceptibility of unselected generations, due to non-exposure to both insecticides for 11 generations, was increased to 1.1-fold in F11 for malathion and to 1.5-fold in F11 for lambda-cyhalothrin. Acetylcholinestrase (AChE) activity increased gradually in both selected and unselected generations until the 11th generation. The activity of AChE in generations (F1-F7) selected with malathion was significantly lower than that of the lambda-cyhalothrin selected and unselected generations. General esterase activity increased 3.7 and 3.0-fold (malathion) and 4.2 and 3.6-fold (lambda-cyhalothrin) compared with the susceptible strain (LS-CP), when either α-napthyl acetate (NA) or β-NA were used as general substrates in F11 selected generations, respectively. A significant increase in glutathione-S-transferase (GST) activity was noticed in the F11 generation recording 6400 and 8800 μmoL min-1 mg-1 protein for malathion-and lambda-cyhalothrin-selected generations, respectively. The ratios recorded in the 11th generations were 3.6 and 4.9 fold as compared with LS-CP for malathion and lambda-cyhalothrin selected generations, respectively. Our results indicate that the Cx. pipiens mosquito strain from Egypt can increase resistance to malathion and lambda-cyhalothrin if these insecticides are continuously or rotationally used to control this species. Increased resistance is likely to be associated with increased activity of target and metabolic enzyme systems.
PDF Fulltext XML References Citation

How to cite this article

El-Sayed A. El-Sheikh, Mohamed-Bassem A. Ashour, Mohamed M. Aamir and Mohamed M. Gamal, 2014. Monitoring the Effect of Insecticide Selection on Culex pipiens (Diptera: Culicidae) Larval Susceptibility to Malathion and Lambda-Cyhalothrin. Journal of Entomology, 11: 14-24.

DOI: 10.3923/je.2014.14.24

URL: https://scialert.net/abstract/?doi=je.2014.14.24

Leave a Comment

Your email address will not be published. Required fields are marked *