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Journal of Entomology

Year: 2013 | Volume: 10 | Issue: 2 | Page No.: 95-102
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Authors

M. Gokuldas

A.P. Ajaykumar

D. Umadevi

V.S. Binitha

K.M. Ismail

K.A. Rasheed

Keywords

Small neuropeptides of the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH), is one of the important groups of peptides that regulate physiological homeostasis in insects. The adipokinetic neuropeptide of Hieroglyphus banian (Order: Orthoptera; Family: Acrididae) was isolated from corpora cardiaca by HPLC; the chromatographic fractions were examined for adipokinetic activity in the plant bug, Iphita limbata in vivo. Two fractions that peaked at retention times of 9.94 and 11.81 min, respectively were found to be significantly active in mobilization of lipids. One of the biologically active peaks (9.94 min) had identical retention time as that of synthetic Locusta migratoria-Adipokinetic Hormone-I (Locmi-AKH-I). These analyses confirmed the presence of Locmi-AKH-I in the corpora cardiaca of H. banian. This data can be additional information to aid in the construction of phylogenetic trees of the insect order Orthoptera, by means of computer programme and protein parsimony algorithms.
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