
	Journal of Entomology
	Year: 2008 \| Volume: 5 \| Issue: 2 \| Page No.: 91-102 DOI: 10.3923/je.2008.91.102

Evaluation of Crude and Fractionated Gut Extract Antigens for Protection Against Camel Tick Hyalomma dromedarii (Acari: Ixodidae)

Sobhy Abdel-Shafy, Mona S. Mahmoud and Mohamed M. Abdel-Aziz

Abstract: The study aim to evaluate crude and two fractionated gut extract antigens of engorged females of Hyalomma dromedarii for protection against tick-feeding. To obtain the two fractionated gut extract antigens (FGE₁ and FGE₂) from Crud Gut Extract (CGE), gel filtration was used. CGE, FGE₁and FGE₂ were used as immunogens for rabbits with Freund`s adjuvant. Six immunized and two control groups were challenged with adult and larvae of H. dromedarii (3 immunized and 1 control for each stage). Results showed that the efficacy of immunogens against adult (E%) showed that FGE₁ recorded the highest immune effects on the adults (64.97%) followed by CGE which recorded (30.97%) and the lowest protection recorded with FGE₂ (21.57%), while E% of CGE, FGE₁ and FGE₂were 85.01, 82.13 and 70.1%, respectively, on larvae. The results of Enzyme Linked Immunuosorbent Assay (ELISA) revealed that the level of antibodies for all tested antigens increased gradually until the date of challenge with ticks and then declined. Western blot technique for both adults and larvae showed that the antigen CGE revealed two reactive bands at molecular weights of 50 and 34 kDa before challenge with ticks. These bands still to be reactive after challenge with ticks. Meanwhile, before challenge, the antigen FGE₁ exhibited 3 reactive bands with molecular weights 145.89, 61.09 and 24 kDa and one band at molecular weight of 34 kDa after challenge with ticks. Moreover, the antigen FGE₂ had only one reactive band at molecular weight of 34 kDa before challenge with ticks and two reactive bands at molecular weight of 34 and 24 kDa after challenge with ticks.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Detection of Toxoplasma gondii (Apicomplexa: Sarcocystidae) in the Brown Dog Tick Rhipicephalus sanguineus (Acari: Ixodidae) Fed on Infected Rabbits

How to cite this article:

Sobhy Abdel-Shafy, Mona S. Mahmoud and Mohamed M. Abdel-Aziz , 2008. Evaluation of Crude and Fractionated Gut Extract Antigens for Protection Against Camel Tick Hyalomma dromedarii (Acari: Ixodidae). Journal of Entomology, 5: 91-102.

DOI: 10.3923/je.2008.91.102

URL: https://scialert.net/abstract/?doi=je.2008.91.102

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		SCIMAGO
		Thomson ISI

Science Alert