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Journal of Entomology
  Year: 2008 | Volume: 5 | Issue: 4 | Page No.: 224-238
DOI: 10.3923/je.2008.224.238
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A New Ecdysiotropic Factor in Adult Male Crickets

S. Reza Kazemi Nezhad and Franz Romer

Oenocytes of the cricket, Gryllus bimaculatus, were characterized by a continuously high secretion of ecdysteroids over several hours. A factor extracted from heads of the male adults caused the oenocytes to enhance immunoreactive ecdysteroid secretion in vitro. This factor was isolated by high performance size exclusion chromatography. Its molecular weight ranged from 26.5 to 30.0 kDa. The partially purified bioactive substance stimulates the ecdysteroid synthesis of oenocytes in a time-and dose-dependent manner. The ecdysiotropin was susceptible to protease degradation and furthermore showed resistance to boiling and alkylation. Exopeptidase did not affect the activity of the peptidergic factor, suggesting that N- and C-terminus are protected. Treatments with dithiothreitol and neuraminidase suggest that disulfide bridge and carbohydrates are necessary for the biological activity of the oenocyte-stimulating ecdysiotropic factor. This study is the first evidence that a factor of head adult insect increased in vitro ecdysteroid secretion in oenocytes that are epidermal in origin.
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How to cite this article:

S. Reza Kazemi Nezhad and Franz Romer, 2008. A New Ecdysiotropic Factor in Adult Male Crickets. Journal of Entomology, 5: 224-238.

DOI: 10.3923/je.2008.224.238






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