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Journal of Entomology
  Year: 2007 | Volume: 4 | Issue: 3 | Page No.: 167-180
DOI: 10.3923/je.2007.167.180
 
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Sequence Analysis of the Ribosomal DNA ITS2 Region for Phlebotomus papatasi (Diptera: Psychodidae)
Omar Hamarsheh, Wolfgang Presber , Ziad Abdeen , Samer Sawalha , Ahmad Al-Lahem and Gabriele Schonian

Abstract:
We investigated the heterogeneity of the ribosomal internal transcribed spacer 2 (ITS2) within 131 individuals of Phlebotomus papatasi from 25 geographic localities in 10 countries by sequencing the PCR-amplified copies of this spacer. Sequence heterogeneity was observed in both sexes from all localities. There are many microsatellite sequences that show insertion-deletion polymorphisms. The length of the ITS2 ranged from 340 to 512 bp and the average GC content of the ITS2 was 29.2%. The phylogenetic relationships among the members of P. papatasi were inferred by neighbour-joining analysis. Although the relationships among P. papatasi populations could not be completely resolved, the phylogenies were in good agreement with previous enzyme electrophoresis studies.
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How to cite this article:

Omar Hamarsheh, Wolfgang Presber , Ziad Abdeen , Samer Sawalha , Ahmad Al-Lahem and Gabriele Schonian , 2007. Sequence Analysis of the Ribosomal DNA ITS2 Region for Phlebotomus papatasi (Diptera: Psychodidae). Journal of Entomology, 4: 167-180.

DOI: 10.3923/je.2007.167.180

URL: https://scialert.net/abstract/?doi=je.2007.167.180

 
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