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Journal of Entomology
  Year: 2006 | Volume: 3 | Issue: 1 | Page No.: 89-94
DOI: 10.3923/je.2006.89.94
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Effects of Dengue 2 Virus Inoculation on Toxorhynchites splendens Larvae

Norazizah Shafee and Sazaly AbuBakar

Potential occurrence of dengue 2 virus (DENV-2)-induced apoptosis in mosquito larvae was investigated. Heads of Toxohrynchites splendens mosquito larvae were inoculated with DENV-2 inoculum at multiplicity of infection of 0.0005-0.001 plaque forming unit per cell. After 7 days of incubation, the larvae heads were severed and squashed onto slides. Following immunofluorescence staining with mouse polyclonal antibody against DENV-2, antigens were detected in 17.5% of the total cells counted. Counterstaining of the cells using colorimetric apoptotic staining method revealed that 21.7% of the antigen-positive cells were in apoptotic state. Statistical analyses of the data showed that the induction of apoptosis was directly correlated to the DENV-2 infection and not merely a metamorphic programmed cell death response.
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How to cite this article:

Norazizah Shafee and Sazaly AbuBakar , 2006. Effects of Dengue 2 Virus Inoculation on Toxorhynchites splendens Larvae. Journal of Entomology, 3: 89-94.

DOI: 10.3923/je.2006.89.94






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