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Journal of Biological Sciences

Year: 2021 | Volume: 21 | Issue: 1 | Page No.: 29-37
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Authors

B.M. Popoola

B.M. Popoola

C.T. Olateru

Keywords

Research Article

Purification and Kinetics of Lipase of Pseudomonas fluorescens from Vegetable Oil Polluted Soil

B.M. Popoola B.M. Popoola's LiveDNA and C.T. Olateru
Background and Objective: Bacterial lipases occupy a place of prominence among biocatalysts and are used for various biotechnological processes. This study was designed to observe the pure lipases and to enable the establishment of the structure-function relationships. Materials and Methods: The lipase of Pseudomonas fluorescens was partially purified using ammonium sulphate, dialysis, column and ion-exchange chromatography. The purity and molecular weight of lipase was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and their kinetics was studied. Results: The enzyme was purified 8.005-fold from Pseudomonas fluorescens. The molecular mass of the partially purified Pseudomonas fluorescens lipase was 45 KDa. Enzyme kinetics displayed Michaelis-Menten constant Km 1.25 mg mL–1 and Vmax value of 0.7 μg sec–1. Conclusion: Hence, knowledge of the kinetics, molecular weights of purified lipases, as well as the three-dimensional structure of lipases plays an important role in designing and engineering lipases for specific purposes.
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