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Journal of Biological Sciences

Year: 2020 | Volume: 20 | Issue: 2 | Page No.: 94-99
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Authors

Normah Awang

Normah Awang

Nur Nadzirah Mohd Nasir

Nurul Farahana Kamaludin

Nurul Farahana Kamaludin

Nur Faizah Abu Bakar

Keywords

Background and Objective: Antimicrobial resistance has become a critical issue to healthcare institutions globally. The search for new and safer antimicrobial agents from natural resources has gained attention of many researchers nowadays. Antimicrobial activity of Tinospora crispa stem extracts on 6 bacterial species and 3 fungal species was evaluated. Materials and Method: Bioactive compounds from T. crispa stem were extracted using 2 methods, maceration and sonication, and using 3 different solvents: chloroform, methanol and water. Antimicrobial activity was determined by disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results: Results showed that sonication yielded a slightly higher percentage of crude extracts for all solvents compared to maceration (The percentage of extract from maceration and sonication methods increased with increasing solvent polarity (chloroform<methanol<water). From the tests, the methanol extract had an MIC value of 100 mg mL–1 against S. aureus and B. cereus and an MBC value of 5.0 mg mL–1 against S. aureus. No activity was shown on the 3 tested fungal species. Conclusion: Hence it was concluded that crude extract of T. crispa stem showed a weak activity of inhibiting bacterial growth and did not inhibit the growth of fungus.
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