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Journal of Biological Sciences

Year: 2020 | Volume: 20 | Issue: 2 | Page No.: 65-72
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Authors

Yahdiana Harahap

Yahdiana Harahap

Wiwin Widayanti

Sunarsih

Keywords

Research Article

Analysis of Acrylamide in Dried Blood Spot of Students by Liquid Chromatography-Tandem Mass Spectrometry

Yahdiana Harahap Yahdiana Harahap's LiveDNA, Wiwin Widayanti and Sunarsih
Background and Objective: Acrylamide is a chemical which is genotoxic, potentially carcinogenic to humans. The aim of this study was to know whether there is a significant difference in the concentration of acrylamide between the DBS samples of the students and the negative control subjects. Materials and Methods: In this research, analysis of acrylamide was performed in dried blood spot (DBS) samples of 15 students, who consume a lot of acrylamide-containing foods and 15 subjects of a negative control group. DBS samples were extracted by a method of ultrasound-assisted liquid extraction and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A reversed-phase chromatography separation was performed on an Acquity UPLC BEH C18 column eluted at a flow rate of 0.2 mL min–1 under a gradient of the mobile phase of 0.1% formic acid and acetonitrile within 3 min. The bioanalytical method of acrylamide in DBS samples of students with propranolol as the internal standard using LC-MS/MS had been validated in this study. Results: The calibration curve range for acrylamide obtained was 2.5-100 μg mL–1 with the coefficient of correlation of more than 0.99. The analysis results of 15 students showed acrylamide levels were within the range of 5.87-14.17 μg mL–1. The DBS samples of the negative control group presented the presence of acrylamide in concentration ranging from 2.72-3.51 μg mL–1. Conclusion: The results indicated that DBS could be used to determine acrylamide exposure in humans and there was a significant difference in acrylamide concentration between DBS of students and negative control subjects.
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