Background and Objective: Asiatic of lily is an important hybrid for trade in the international market because of its colour, beauty and long vase life. The objective of study was to establish a micropropagation protocol for Asiatic hybrid lily using bulb scales for continuing in vitro cultures. Methodology: Sterilized treatments were investigated at three concentrations (0.05, 0.1 and 0.2%) of HgCl2 (MC). The MS medium supplemented with various concentrations (0, 0.5, 1, 1.5 and 2.0 mg L1) of BA (6-Benzylaminopurine) and 2ip (isopentyl adenine) was used for in vitro scales multiplication. For bulblets induction, 0.5 mg L1 of both BA and thidiazuron (TDZ) each alone or combined with α-naphthalene acetic acid (NAA) at 1.0 and 2.0 mg L1 were examined. Different parameters were statistically analyzed using randomized complete block design. Results: The highest percentage of both free contaminated explants and survival (88.89 and 77.77%, respectively) were clearly appeared when 10% of sodium hypochlorite and mercuric chloride at 0.1% were used. Using MS (Murashige and Skoog, 1962) culture medium supplemented with different concentrations of BA or 2ip had a significant promotion effect on the number of bulbscales/explant which can induce adventitious bulblet formation and use for micropropagation. MS culture medium supplemented with TDZ at 0.5 mg L1+NAA (2.0 mg L1) was favored for obtaining the highest numbers of shoots, leaves and bulblets induced/explant (9.33, 4.67 and 9.33, respectively). IAA at 0.5 mg L1 was favored for rooting percentage, number of roots as well as number of formed bulblets/shootlet (100%, 2.5 and 3.33, respectively). Most of plants were easily grow, normally acclimatized to the green house conditions and 98% of them were survived. Conclusion: Tissue culture technique is suitable propagation method for producing great new bulbs of Asiatic hybrid lily plant. PDFFulltextXMLReferencesCitation
How to cite this article
Lobna Salah Taha, Sawsan Sami Sayed, Mahmoud Mohamed Farahat and Iman Mohamed El-Sayed, 2018. In vitro Culture and Bulblets Induction of Asiatic Hybrid Lily Red Alert. Journal of Biological Sciences, 18: 84-91.