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Journal of Biological Sciences

Year: 2018 | Volume: 18 | Issue: 2 | Page No.: 84-91
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Authors

Lobna Salah Taha

Lobna Salah Taha

Sawsan Sami Sayed

Mahmoud Mohamed Farahat

Iman Mohamed El-Sayed

Keywords

Background and Objective: Asiatic of lily is an important hybrid for trade in the international market because of its colour, beauty and long vase life. The objective of study was to establish a micropropagation protocol for Asiatic hybrid lily using bulb scales for continuing in vitro cultures. Methodology: Sterilized treatments were investigated at three concentrations (0.05, 0.1 and 0.2%) of HgCl2 (MC). The MS medium supplemented with various concentrations (0, 0.5, 1, 1.5 and 2.0 mg L–1) of BA (6-Benzylaminopurine) and 2ip (isopentyl adenine) was used for in vitro scales multiplication. For bulblets induction, 0.5 mg L–1 of both BA and thidiazuron (TDZ) each alone or combined with α-naphthalene acetic acid (NAA) at 1.0 and 2.0 mg L–1 were examined. Different parameters were statistically analyzed using randomized complete block design. Results: The highest percentage of both free contaminated explants and survival (88.89 and 77.77%, respectively) were clearly appeared when 10% of sodium hypochlorite and mercuric chloride at 0.1% were used. Using MS (Murashige and Skoog, 1962) culture medium supplemented with different concentrations of BA or 2ip had a significant promotion effect on the number of bulbscales/explant which can induce adventitious bulblet formation and use for micropropagation. MS culture medium supplemented with TDZ at 0.5 mg L–1+NAA (2.0 mg L–1) was favored for obtaining the highest numbers of shoots, leaves and bulblets induced/explant (9.33, 4.67 and 9.33, respectively). IAA at 0.5 mg L–1 was favored for rooting percentage, number of roots as well as number of formed bulblets/shootlet (100%, 2.5 and 3.33, respectively). Most of plants were easily grow, normally acclimatized to the green house conditions and 98% of them were survived. Conclusion: Tissue culture technique is suitable propagation method for producing great new bulbs of Asiatic hybrid lily plant.
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