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Journal of Biological Sciences
  Year: 2018 | Volume: 18 | Issue: 5 | Page No.: 216-222
DOI: 10.3923/jbs.2018.216.222
Characterization of Putative Lipase Gene from Alcaligenes sp. JG3 Bacterium via Cloning
Norman Yoshi Haryono, Winarto Haryadi and Tri Joko Raharjo

Abstract:
Background and Objective: Alcaligenes sp. JG3 is an endemic bacterium from Indonesia, isolated from cultivated cornfield of Central Java. This bacterium is able to produce lipase with fairly high activity. This study aimed to determine the complete sequence of the lipase gene using cloning method. Materials and Methods: The primer pair used in this study (primer forward 5’-ATGACCGAGCTGACTGTAG-3’ and reverse 5’-TCAGGAGGGGTAAATCCAC-3’) were designed based on lipase gene from Alcaligenes faecalis sub sp. faecalis NCIB 8687 and the gene was cloned using pGEM-T Easy Vector. The nucleotide sequence was analyzed using online software such as CLUSTAL Omega, BLASTp, ExPASy and Conseq Server. Results: The complete lipase JG3 gene sequence consists of 357 amino acids from ORF to the stop codon. Lipase JG3 had high similarity to other lipase from genus Alcaligenaceae which was up to 99% and also possessed some characteristic of ABC transporter protein. Conclusion: The designed primer pair based on lipase gene from Alcaligenes faecalis were able to amplify 1071 bp lipase gene of Alcaligenes sp. JG3 and the gene was successfully cloned into Escherichia coli.
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  •    Characterization of Lipase Gene Fragment from Alcaligenes sp. JG3 Bacterium
How to cite this article:

Norman Yoshi Haryono, Winarto Haryadi and Tri Joko Raharjo, 2018. Characterization of Putative Lipase Gene from Alcaligenes sp. JG3 Bacterium via Cloning. Journal of Biological Sciences, 18: 216-222.

DOI: 10.3923/jbs.2018.216.222

URL: https://scialert.net/abstract/?doi=jbs.2018.216.222

 
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