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Journal of Biological Sciences

Year: 2018 | Volume: 18 | Issue: 3 | Page No.: 107-114
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Authors

Reham Abdel-aal Awad Morsy

Reham Abdel-aal Awad Morsy

Salwa Mahmoud Sarhan

Laila Ahmed Rashed

Mohamed Gomaa Attia-Zouair

Mohamed Mahmoud Ahmed

Mona Mahmoud El-Batran

Nadia Lashin Soliman

Keywords

Background and Objective: Many lines of evidence suggested that the oxidative stress is important in pathogenesis of diseases. Nicotine inhibited the proliferation and osteoblastic differentiation of mesenchymal stem cells (MSCs) and decrease alkaline phosphatase (ALP) activity. The biological antioxidants, such as vitamin E act in synergy to form an integrated network of antioxidant defense, which is considered to be the first line of defense against any increase in the production of reactive oxygen species (ROS). The objective of the study was to investigate the effect of vitamin E on the proliferation as well as differentiation potentiality of rat’s dental follicle stem cells (DFSCs) treated with nicotine. Materials and Methods: Rat DFSCs were isolated, cultured and divided into four groups. The 1st group was the control, the 2nd group was cultured with 5 mM of nicotine, the 3rd group was cultured with 2mM of vitamin E (1h) prior to nicotine treatment and the 4th group was cultured with 2 mM of vitamin E (1 h) after nicotine treatment. Methyl thiazol tetrazolium (MTT) assay was used to assess the cell viability among the groups used. Wright staining solution was used to assess the effects of nicotine on cell morphology. The flow cytometric analysis was used to identify the stemness of isolated rat’s DFSCs by CD34 and CD29. Alizarin red-sulfate (AR-S) staining and quantitative real time-polymerase chain reaction (qRT-PCR) utilizing alkaline phosphatase (ALP) expression and activity were used to determine the osteoblastic differentiation. Results: The results of this study revealed that increase the proliferation rate and the ALP expression and activity in group I followed by group III and IV then group II. The vacuolization and loss of cell membrane of DFSCs appeared to be more extensive in group II compared to group III and group IV. Conclusion: These findings indicated that vitamin E could relief the state of oxidative process induced by nicotine. Vitamin E also can increase the capability of DFSCs to proliferate and differentiate into osteogenic lineage detected by AR-S stain and increase the ALP level.
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Reham Abdel-aal Awad Morsy, Salwa Mahmoud Sarhan, Laila Ahmed Rashed, Mohamed Gomaa Attia-Zouair, Mohamed Mahmoud Ahmed, Mona Mahmoud El-Batran and Nadia Lashin Soliman, 2018. Influence of Vitamin E on Proliferation and Differentiation of Rat’s Dental Follicle Stem Cells Treated with Nicotine (An Experimental Study). Journal of Biological Sciences, 18: 107-114.

DOI: 10.3923/jbs.2018.107.114

URL: https://scialert.net/abstract/?doi=jbs.2018.107.114

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