
	Journal of Biological Sciences
	Year: 2017 \| Volume: 17 \| Issue: 2 \| Page No.: 61-68 DOI: 10.3923/jbs.2017.61.68

In vitro α-amylase and α-glucosidase Inhibitory Activities of Coccinia grandis Aqueous Leaf and Stem Extracts

Pawitra Pulbutr, Natcha Saweeram, Thitiworada Ittisan, Hattaya Intrama, Achida Jaruchotikamol and Benjamart Cushnie

Abstract: Background: Coccinia grandis (ivy gourd) an edible plant widely consumed in many Southeast Asian countries has been used traditionally for the treatment of diabetes mellitus. However, its role in the regulation of carbohydrate-digesting enzymes, especially α-amylase and α-glucosidase has not been clearly established. Materials and Methods: The aqueous leaf and stem extracts of Coccinia grandis (the CGL and the CGS extracts, respectively) were prepared and their actions toward in vitro activities of α-amylase and α-glucosidase were examined. The activities of α-amylase and α-glucosidase were indicated by the amounts of maltose and p-nitrophenol generated in the reactions, respectively. Results: The CGL and the CGS extracts possessed an α-amylase inhibitory action with the IC_50s of 8.09±0.72 and 8.06±1.27 mg mL^–1, respectively. The CGL extract produced a mixed inhibition against α-amylase, whilst the CGS extract inhibited the enzyme in an uncompetitive manner. The CGL and the CGS extracts exhibited an inhibitory action against α-glucosidase enzyme with the IC_50s of 77.66±9.16 μg mL^–1 and 0.75±0.11 mg mL^–1, respectively. Both extracts acted as mixed enzyme inhibitors against α-glucosidase. Conclusion: These results support the traditional use of Coccinia grandis in diabetic patients, especially for the control of postprandial plasma glucose level. However, further studies on identification of the active phytochemicals acting as enzyme inhibitors and in vivo carbohydrate-digesting enzyme inhibition are required to verify its potential clinical use in diabetes mellitus.

PDF Fulltext XML References Citation Report Citation

How to cite this article:

Pawitra Pulbutr, Natcha Saweeram, Thitiworada Ittisan, Hattaya Intrama, Achida Jaruchotikamol and Benjamart Cushnie, 2017. In vitro α-amylase and α-glucosidase Inhibitory Activities of Coccinia grandis Aqueous Leaf and Stem Extracts. Journal of Biological Sciences, 17: 61-68.

DOI: 10.3923/jbs.2017.61.68

URL: https://scialert.net/abstract/?doi=jbs.2017.61.68

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRICOLA
		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		EMBASE
		Google Scholar
		SCIMAGO

Science Alert