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Journal of Biological Sciences
  Year: 2017 | Volume: 17 | Issue: 7 | Page No.: 334-346
DOI: 10.3923/jbs.2017.334.346
 
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Newly Formulated Antioxidant Rich Dietary Supplement in Jelly Form for Alleviation of Liver Diseases in Rats

Maha Hanafy Mahmoud , Hany Mohamed Ahmed Wahba , Marwa Hanafy Mahmoud and Ibrahim Hassan Badawy

Abstract:
Background and Objective: Nonalcoholic fatty liver disease (NAFLD) is the major cause of chronic liver disease worldwide. The present study aimed to prepare a dietary supplement rich in antioxidants that is able to prevent nonalcoholic fatty liver associating metabolic disorders and to limit its progression into steatohepatitis. Materials and Methods: This dietary supplement is in jelly form and composed of prunes, dried grapes, carrots, cape gooseberry, pomegranate peel, chicory, sumac, sweet corn, germinated soybeans and vitamins B12, C and D. HPLC analysis and the antioxidant activity for the formulated dietary supplement were carried out. A biological experiment was done on 30 male Wistar rats categorized into 5 groups for 4 weeks after nonalcoholic fatty liver induction by feeding rats high fat and cholesterol (HFC) diet with bile acids for 8 weeks. Biochemical analysis and histopathological examination were done. The one-way ANOVA test was done followed by Duncan test. Results: The supplement antioxidant activity was relatively high and HPLC analysis revealed the presence of several polyphenolic compounds. Results revealed an altered lipid profile, an elevation in activity of plasma transaminases, lipid peroxides and tumor necrosis factor alpha (TNF-α), while there was a reduction in total protein, albumin and activity of antioxidant enzymes for HFC fed group. Adding the formula to the diet of the HFC fed rats nearly normalized the aforementioned parameters. Histopathological examination reinforced the biochemical findings. Conclusion: The obtained data proved that the prepared dietary supplement managed to restore the metabolic changes accompanying fatty liver and to limit its progression into steatohepatitis.
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How to cite this article:

Maha Hanafy Mahmoud, Hany Mohamed Ahmed Wahba, Marwa Hanafy Mahmoud and Ibrahim Hassan Badawy, 2017. Newly Formulated Antioxidant Rich Dietary Supplement in Jelly Form for Alleviation of Liver Diseases in Rats. Journal of Biological Sciences, 17: 334-346.

DOI: 10.3923/jbs.2017.334.346

URL: https://scialert.net/abstract/?doi=jbs.2017.334.346

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