Submit Manuscript

Journal of Biological Sciences

Year: 2017 | Volume: 17 | Issue: 1 | Page No.: 1-10
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 114

Authors

Nanung Agus Fitriyanto

Nanung Agus Fitriyanto

Atik Winarti

Fatih Akbar Imara

Yuny Erwanto

Takashi Hayakawa

Tomoyuki Nakagawa

Keywords

Background and Objective: A proper management processes for safe and efficient handling of animal manure is important to minimize the possibilities of global environmental problem due to the emission of unpleasant odor. This study was performed to identify strains bacteria screened from the soil in the odorous region of apoultry format Yogyakarta city, Indonesia, to evaluate their capability for growing in ammonium and animal’s urine high content medium and also to investigate the ability to reduce ammonium concentration from the liquid medium. Materials and Methods: The strain is originally obtained from soil sample collected at ammonia high-emitted of chicken hen production area at tropical country Indonesia. The isolation was using a 1/100 nutrient agar with high concentration of (NH4)2SO4 as an organic ammonium stressor. Selected strain was then identified based on morphology and biochemical identifications, as well as molecular identification. The reduction of ammonium was measured from cultured medium using nessler reagent spectroscopically. Results: High ammonium sulfate-responsive, strain LS3K was successfully isolated and identified based on morphology, physiology and biochemical identifications, as well as molecular identification based on 16S rRNA sequence. Based on the morphology, physiological observation and the 16S rRNA gene sequence, strain LS3T was identified as Pseudomonas spp. The content of DNA G+C of the strain was 55.2%. The 16S rRNA gene sequence analysis demonstrated that the isolate is showing high similarity to species of Pseudomonas tolaasii. The cells of strain LS3K are short rods, aerobic and motile. Colonies (l-2 mm in diameter) on plate culture are yellowish, circular and smooth. This bacterium has an optimal growth temperature 30°C. Conclusion: Strain LS3K can tolerate ammonium at 756.77 mM and has the ability for growing in animal’s manure contained a medium that should make it applicable for use in animal waste handling systems as well as for enhancing the capability of deodorization. Ammonium was oxidized 5.648 mg L–1 by strain LS3K during 30 h cultivation period.
PDF Fulltext XML References Citation

How to cite this article

Nanung Agus Fitriyanto, Atik Winarti, Fatih Akbar Imara, Yuny Erwanto, Takashi Hayakawa and Tomoyuki Nakagawa, 2017. Identification and Growth Characters of Nitrifying Pseudomonas sp., LS3K Isolated from Odorous Region of Poultry Farm. Journal of Biological Sciences, 17: 1-10.

DOI: 10.3923/jbs.2017.1.10

URL: https://scialert.net/abstract/?doi=jbs.2017.1.10

Leave a Comment

Your email address will not be published. Required fields are marked *