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Journal of Biological Sciences

Year: 2016 | Volume: 16 | Issue: 6 | Page No.: 236-241
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Authors

Nantana Jangsuwan

Parisatcha Sangsuwan

Keywords

Research Article

Antiproliferation of Fibrosarcoma Cell Line (L929) by Hua-Khao-Yen-Tai Thai Medicinal Plant (Dioscorea membranacea Pierre)

Nantana Jangsuwan and Parisatcha Sangsuwan
Background: Extracts of many Thai medicinal plants affected anti-proliferation of cancer cell line. This study was to determine cytotoxic activity and apoptotic gene expression of fibrosarcoma cell line (L929) treated with Thai medicinal plant, Dioscorea membranacea Pierre. Materials and Methods: The ethanolic crude extracts of Hua-Khao-Yen-Tai (Dioscorea membranacea Pierre) were tested for anti-proliferative activity, apoptotic gene expression and protein expression of L929 by using MTT assays, RT-PCR and Western blot analysis. Results: The MTT assays showed 50% of viability at 15 μg mL–1 after treatment for 24 h and 25 μg mL–1 at 48 h of L929. The L929 cells showed character of apoptosis: Cell shrinkage, membrane blebbing and nuclear condensation when treated with Hua-Khao-Yen-Tai ethanolic extracts. The expression level of BAX gene increased more than control but Bcl-2 decreased lower than control. The results of Western blot analysis showed up-regulation of BAX protein and down-regulation of Bcl-2 protein when compared with control cells at 24 and 48 h. Conclusion: Results indicated that the ethanolic crude extracts of Hua-Khao-Yen-Tai induced apoptosis and anti-proliferation on fibrosarcoma cell line. This could be beneficial to the further development of cancer treatments in the future.
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