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Journal of Biological Sciences

Year: 2016 | Volume: 16 | Issue: 6 | Page No.: 221-228
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Authors

Raviteja Pagolu

Md. Shafiqul Islam Sikdar

Jung-Sup Kim

Keywords

Research Article

Characterization and Functional Complementation with an Inhibitor of Rice Chorismate Synthase

Raviteja Pagolu, Md. Shafiqul Islam Sikdar and Jung-Sup Kim
Background: Chorismate synthase (EC 4.6.1.4) is the last enzyme synthesizing chorismate which is the branch point for synthesis of three aromatic amino acids in shikimate pathway in bacteria and plants. Materials and Methods: A gene encoding chorismate synthase from rice (OsCS) was analyzed and characterized by bioinformatics and molecular genetics approaches in functional complementation. Results: The analysis of a cDNA sequence revealed that the open reading frame consisted of 438 amino acid residues with approximate molecular weight of 46.9 kDa which showed high homology with related sequences from bacteria and plants. The rice OsCS was expressed in an aroC mutant of Escherichia coli strain, which was able to complement the mutant in the absence of tryptophan and inhibited the chorismate synthase activity by its inhibitor, bathophenanthroline. Conclusion: These results indicate that OsCS encodes chorismate synthase and suggest the possible application in herbicide development.
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