Characterization of a Gene Encoding 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Rice

Research Article

Characterization of a Gene Encoding 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Rice

Raviteja Pagor, Md. Shafiqul Islam Sikdar, Eun-Sung Moon and Jung-Sup Kim

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS) is an entry enzyme of the shikimate pathway which is responsible for primary carbohydrate metabolism with the biosynthesis of most aromatic amino acids in microorganisms and plants. Enzymes of the pathway are drawing attention in the recent years because they are recognized as potential targets for design of antimicrobial drugs and herbicides. Analysis of sequences from rice revealed that the OsDAHPS showed a full-length open reading frame consisting of 537 amino acids, which encoded for a protein of approximately 59.0 kDa. The predicted amino acid sequence of OsDAHPS is highly homologous to those of DAHPS enzymes from many plants. The OsDAHPS expression in a aroH mutant of Escherichia coli showed that the gene was functionally capable of complementing the mutant. These results showed that the OsDAHPS encoded for a protein in 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in rice.

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Raviteja Pagor, Md. Shafiqul Islam Sikdar, Eun-Sung Moon and Jung-Sup Kim, 2015. Characterization of a Gene Encoding 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Rice. Journal of Biological Sciences, 15: 33-39.

DOI: 10.3923/jbs.2015.33.39

URL: https://scialert.net/abstract/?doi=jbs.2015.33.39

Journal of Biological Sciences

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Authors

Raviteja Pagor

Md. Shafiqul Islam Sikdar

Eun-Sung Moon

Jung-Sup Kim

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Research Article

Characterization of a Gene Encoding 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Rice

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