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Acute Effect of Nanosilver to Function and Tissue Liver of Rat after Intraperitioneal Injection |
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Monir Doudi and Mahbubeh Setorki |
Abstract:
The use of nanosilver particles in medicine, due to its antibacterial
and antifungal characteristics, dates back hundreds of years. However, several
studies indicate that besides the antibacterial characteristics, these nanoparticles
have high toxic effects on mammalian cells. For this study, 32 Wistar male rats
were obtained. After 2 weeks of accommodation, they were randomly divided into
groups; three nanosilver-treated rat groups and one Control Group (CG). The
three treated groups were exposed to 5 cc of solution containing 5, 10 and 100
ppm nanosilver(Ag) (group 1, 2 and 3, respectively) via IP injection for 7 successive
days. The control group was treated with 5 cc of normal saline solution with
the same procedure. This study was performed to investigate the effect of different
nanosilver concentrations on biochemical parameters such as Serum Glutamic Oxaloacetic
Transaminase (SGOT) and Serum Glutamic-pyruvic Transaminase (SGPT) at various
time points (2, 7 and 14 days). After 14 days, the tissue of liver and lung
were collected and investigated. There was no significant difference between
SGPT and SGOT liver enzymes in different groups (p>0.005). However, the amount
of SGOT enzyme had increased in the treatment groups compared to the control
group but this difference was not significant. Liver histological findings in
this study showed no pathological changes except the decrease in acidophilic
property in treatment group 1; reduction in acidophilic property, hepatocyte
hypertrophy, full sinusoids fading and hyperemia of the central veins in treatment
group 2 and reduction of acidophilic properties, hepatocyte hypertrophy, fading
and thinning of sinusoids and slightly enlarged central veins in treatment group
3. The histological findings of the lung in the group receiving a 10 ppm concentration
of nanosilver were complete destruction of air sacs and bronchioles and disfiguring
of the lung tissues from their normal form. Moreover, in the group receiving
a concentration of 100 ppm they were sever and clear hyperemia, disfiguring
of the air sacs from their normal form, abnormal condition of the bronchioles,
vessel rupture and blood leakage to the bronchioles and air sacs. The histological
findings in the group receiving a concentration of 300 ppm were complete destruction
of air sacs and bronchioles and obvious hyperemia in the tissue (which indicates
the impact of nanoparticles in this tissue, deformity in some places were so
much that it was not possible to identify lung tissue). According to the findings
it can be concluded that the nanosilver particles, which were used in the form
of solution in citrate stabilizer, with spherical shape and 10 nm in size caused
complete destruction of lung tissue and partially destroyed liver tissue.
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How to cite this article:
Monir Doudi and Mahbubeh Setorki, 2014. Acute Effect of Nanosilver to Function and Tissue Liver of Rat after Intraperitioneal Injection. Journal of Biological Sciences, 14: 213-219.
DOI: 10.3923/jbs.2014.213.219
URL: https://scialert.net/abstract/?doi=jbs.2014.213.219
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