Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Journal of Biological Sciences
  Year: 2014 | Volume: 14 | Issue: 2 | Page No.: 134-141
DOI: 10.3923/jbs.2014.134.141
Molecular Characterization and Phylogenetic Analysis of Selected Pigeon Paramyxovirus Type-1 (PPMV-1) Indian Isolates
K.A. Naveen, S.D. Singh, J.M. Kataria, R. Barathidasan and K. Dhama

Abstract:
Newcastle Disease (ND) is a highly contagious infection of poultry which manifest in a wide range of severity from subclinical infection to lethal disease. In the past, a number of Newcastle disease outbreaks in poultry and other bird species have been ascribed to pigeon paramyxovirus type-1 (PPMV-1) infection. The conventional in vivo pathogenicity tests to assess the pathogenicity of PPMV-1 viruses have provided equivocal results. Lately, restriction enzyme analysis technique has been employed for unequivocal identification of individual strains of Newcastle Disease Virus (NDV) in poultry. In this study, sequence analysis of the F1/F2 cleavage site of the F gene of APMV-1 isolated from pigeons in India was attempted for pathotype prediction and determination of molecular epidemiology. Six pigeon origin NDV isolated in India between 1991 and 2001 were selected for this study. A portion of NDV F gene including the cleavage site was amplified by Polymerase Chain Reaction (PCR) and sequenced directly. The total number of nucleotide substitution among all six isolates ranged from 6 to 20; whereas, only four amino acid substitutions were observed. Nucleotides at position 304 and 357 were unique to all the pigeon isolates. The cleavage-activation site (380-397) had no nucleotide substitution and all the six pigeon isolates shared the amino acid sequence 112RRQKRF117 as that of velogenic viruses. The results of this molecular characterization study of Indian PPMV-1 isolates would help design better prevention and control measures for this important pathogen.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Novel and Emerging Therapies Safeguarding Health of Humans and Their Companion Animals: A Review
  •    Immunomodulatory and Therapeutic Potentials of Herbal, Traditional/Indigenous and Ethnoveterinary Medicines
How to cite this article:

K.A. Naveen, S.D. Singh, J.M. Kataria, R. Barathidasan and K. Dhama, 2014. Molecular Characterization and Phylogenetic Analysis of Selected Pigeon Paramyxovirus Type-1 (PPMV-1) Indian Isolates. Journal of Biological Sciences, 14: 134-141.

DOI: 10.3923/jbs.2014.134.141

URL: https://scialert.net/abstract/?doi=jbs.2014.134.141

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom