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Journal of Biological Sciences

Year: 2014 | Volume: 14 | Issue: 2 | Page No.: 134-141
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Authors

K.A. Naveen

S.D. Singh

J.M. Kataria

R. Barathidasan

K. Dhama

Keywords

Research Article

Molecular Characterization and Phylogenetic Analysis of Selected Pigeon Paramyxovirus Type-1 (PPMV-1) Indian Isolates

K.A. Naveen, S.D. Singh, J.M. Kataria, R. Barathidasan and K. Dhama
Newcastle Disease (ND) is a highly contagious infection of poultry which manifest in a wide range of severity from subclinical infection to lethal disease. In the past, a number of Newcastle disease outbreaks in poultry and other bird species have been ascribed to pigeon paramyxovirus type-1 (PPMV-1) infection. The conventional in vivo pathogenicity tests to assess the pathogenicity of PPMV-1 viruses have provided equivocal results. Lately, restriction enzyme analysis technique has been employed for unequivocal identification of individual strains of Newcastle Disease Virus (NDV) in poultry. In this study, sequence analysis of the F1/F2 cleavage site of the F gene of APMV-1 isolated from pigeons in India was attempted for pathotype prediction and determination of molecular epidemiology. Six pigeon origin NDV isolated in India between 1991 and 2001 were selected for this study. A portion of NDV F gene including the cleavage site was amplified by Polymerase Chain Reaction (PCR) and sequenced directly. The total number of nucleotide substitution among all six isolates ranged from 6 to 20; whereas, only four amino acid substitutions were observed. Nucleotides at position 304 and 357 were unique to all the pigeon isolates. The cleavage-activation site (380-397) had no nucleotide substitution and all the six pigeon isolates shared the amino acid sequence 112RRQKRF117 as that of velogenic viruses. The results of this molecular characterization study of Indian PPMV-1 isolates would help design better prevention and control measures for this important pathogen.
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