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Journal of Biological Sciences
  Year: 2013 | Volume: 13 | Issue: 6 | Page No.: 514-520
DOI: 10.3923/jbs.2013.514.520
 
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Diversity Analysis of Bacillus thuringiensis Isolates Recovered from Diverse Habitats in India using Random Amplified Polymorphic DNA (RAPD) Markers

Jawahar Katara, Rupesh Deshmukh, Nagendra K. Singh and Sarvjeet Kaur

Abstract:
Bacillus thuringiensis is a bacterium of huge agronomic and scientific interest. The subspecies of this bacterium colonize and kill a large variety of host insects and nematodes with a high degree of specificity. In the present investigation, 32 native isolates of Bacillus thuringiensis recovered from different regions in India and 8 known Bacillus thuringiensis strains were analyzed at the molecular level using random amplified polymorphic DNA (RAPD) markers. Seven RAPD markers were used for diversity analysis. The RAPD banding pattern data was subjected to dendrogram construction using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis using NTSYSpc2.2 software. Eight main clusters were formed at 25% similarity level and four isolates were standalone of these clusters. Most of the isolates were found to be diverse, even though they were isolated from the same source or location. The RAPD markers were found to be effective to distinguish B. thuringiensis native isolates recovered from different sources and locations. The results of present investigation help to understand diversity of B. thuringiensis in India, which would be exploited to find new types of B. thuringiensis endotoxins.
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How to cite this article:

Jawahar Katara, Rupesh Deshmukh, Nagendra K. Singh and Sarvjeet Kaur, 2013. Diversity Analysis of Bacillus thuringiensis Isolates Recovered from Diverse Habitats in India using Random Amplified Polymorphic DNA (RAPD) Markers. Journal of Biological Sciences, 13: 514-520.

DOI: 10.3923/jbs.2013.514.520

URL: https://scialert.net/abstract/?doi=jbs.2013.514.520

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